The immunohistochemical detection of Mycoplasma bovis and bovine viral diarrhea virus in tissues of feedlot cattle with chronic, unresponsive respiratory disease and/or arthritis.

The purpose of this study was to determine the frequency of selected pathogens in the tissues of a group of feedlot cattle with chronic disease (most often respiratory disease and/or arthritis). Samples of lung and joint tissues from 49 feedlot animals that had failed to respond to antibiotic therapy were tested by immunohistochemical staining for the antigens of Mycoplasma bovis, Haemophilus somnus, Pasteurella (Mannheimia) hemolytica, and bovine viral diarrhea virus (BVDV). Mycoplasma bovis was demonstrated in over 80% of cases, including in 45% of joints and 71% of lungs tested. Mycoplasma bovis was the only bacterial pathogen identified in the joints. Haemophilus somnus and Pasteurella (Mannheimia) haemolytica were found in 14% and 23% of cases, respectively, and were confined to the lungs in all instances. Infection with BVDV was demonstrated in over 40% of cases. Mycoplasma bovis and BVDV were the most common pathogens persisting in the tissues of these animals that had failed to respond to antibiotic therapy.     

关于我国海水网箱养鱼发展现状与危机分析及可持续发展的探讨

我国海水网箱养鱼发展现状是以个体联合体生产经营为主体的低技术水平的数量规模化，众多传统港湾已形成了大范围的严重超负荷养殖状态，其对环境巨大的污染力引发鱼病猖獗和频受赤潮侵袭，已面临严峻的养殖环境危机。本文综合分析了我国海水网箱养鱼的发展现状与负面效应及其现状危机，并初步进行了危机根源和可持续发展的讨论分析。     

Enhancement of sulfide auto-oxidation by metal ion in artificial seawater and a seawater which is a possible source of aoshio

SUMMARY: Aoshio is hypoxic milky blue-green seawater observed in some eutrophic bays. Previous studies have shown that colloidal sulfur causes the coloration and that the source of aoshio water is attributed to coastal anoxic bottom water. Occurrences of aoshio have been reported in limited areas of coastal seawater, although hypoxic transparent water seems rather universal. Promotion in auto-oxidation of sulfide by metal ions in seawater was investigated to explain the occurrences of aoshio. Artificial seawater containing 10 μM metal ion was assayed for the sulfide oxidation rate. The velocity constant which represents the oxidation rate within the initial 30 min and the amount of reacted sulfide in 2 h were determined by oxygen monitoring and sulfide quantification, respectively. Fe²⁺ and Cu²⁺ enhanced the initial 30 min reaction. Fe²⁺, Fe³⁺ and Ni²⁺ increased the amount of reacted sulfide in 2 h, forming whitish turbid water. Seawater from a suspected source of aoshio water was also assayed for the auto-oxidation rate of sulfide. The oxidation rate in water from 12 m depth was 13–19 times higher than the artificial seawater without an addition of heavy metal ions. More than 15% of the oxidation rate in 12 m deep seawater was explained by dissolved iron in the seawater.     

槟榔红色素的抗氧化活性

测定槟榔红色素(areca red pigment,AP)对 DPPH·和·OH的清除能力、对脂质体过氧化的抑制能力、对Fe2+的络合能力,并测定其还原能力.结果表明:槟榔色素对DPPH·、·OH的清除能力较强:对脂质体过氧化的抑制能力很强,抑制率高达79.84%;与VE、没食子酸(gallic acid,GA)、BHT相比,槟榔色素的还原力、对Fe2+的络合能力较弱.说明槟榔红色素是较好的抗氧化剂.     

鼠李糖制备新工艺研究

采用正交设计方法探讨发酵法从芦丁水解液里提取制备鼠李糖的最佳制备工艺,优选出鼠李糖纯度达到99%以上的最佳制备工艺为:温度30℃,pH值5.0,酵母量为母液的2%。     

银川地区羊肝中阿苯达唑(丙硫苯咪唑)残留情况的调查

阿苯达唑又称丙硫苯咪唑或丙硫咪唑，有时又称抗蠕敏，是目前国内常用的驱虫效果好、范围广、毒性较低的兽用驱虫药。随着家庭舍饲养羊规模的不断扩大，饲养者为了减少因寄生虫导致的死亡并增加育肥效果，常把阿苯达唑作为育肥前预防驱虫或寄生虫病治疗的首选药，出现了滥用或超剂量使用的现     

播量对水稻生长发育及干物质积累的影响

用盆栽方法进行播量对寒地水稻生长发育及干物质积累影响试验,结果表明:稀播的秧苗发根力强、分蘖多、茎基宽、充实度高。播量200g/m2的产量最高。     

试论工科院校大学生的审美教育

工科院校在强调专业教育的同时，没能同样重视审美教育。加强对工科院校大学生的审美教育，有助于培养正确的审美观，提高审美能力;有助于完善知识结构，提升人文精神; 有助于形成独立完善的人格，提升人格境界;有助于培养个性和谐、德智体美全而发展的高素质人才。     

S1核酸酶突变检测法的优化

优化了S1核酸酶突变检测体系,结果表明(1)扩增法比杂交法产生异源双链DNA更节省时间;(2)PCR产物可以直接作为突变检测的底物;(3)适当降低反应温度、适当增加反应缓冲液中的NaCl浓度、适当缩短反应时间可提高突变检测效果。